ABSTRACT
Objective To explore the relationship between perceived stress and work well-being of medical stuffs,and test the mediating effect of self-efficacy between above two variables.Methods A total of 1 296 doctors from 29 hospitals in Beijing were assessed by work well-being scale,general serf-efficacy scale (GSES) and Chinese Perceived Stress Scale (CPSS).Structural equation modeling (SEM) was conducted to test the mediating effect of self-efficacy on the relationship between perceived stress and work well-being.Results (1) Perceived stress(37.62±7.20) was negatively correlated with general self-efficacy(2.47±0.55),cognitive work well-being (4.16 ± 0.78) and affective work well-being (21.57 ± 5.63) (r =-0.456,P＜0.01;r=-0.406,P＜0.01;r=-0.542,P＜0.01).General serf-efficacy was positively correlated with cognitive work well-being and affective work well-being(r=0.382,P＜0.01;r=0.510,P＜0.01) (2) In the relationship between perceived stress and affective work well-being,general self-efficacy played a partial mediator role (P＜0.01),the mediating effect was 14.5％.In the relationship between perceived stress and cognitive work well-being,general self-efficacy played a partial mediator role (P＜0.01),and the mediating effect was 46.3％.Conclusion Perceived stress of medical stuffs influenced their work well-being through self-efficacy.It is advised to improve the medical stuffs' self-efficacy in order to raise their level of work well-being.
ABSTRACT
Objective To explore the relationship between perceived stress and work well-being of medical stuffs,and test the mediating effect of self-efficacy between above two variables.Methods A total of 1 296 doctors from 29 hospitals in Beijing were assessed by work well-being scale,general serf-efficacy scale (GSES) and Chinese Perceived Stress Scale (CPSS).Structural equation modeling (SEM) was conducted to test the mediating effect of self-efficacy on the relationship between perceived stress and work well-being.Results (1) Perceived stress(37.62±7.20) was negatively correlated with general self-efficacy(2.47±0.55),cognitive work well-being (4.16 ± 0.78) and affective work well-being (21.57 ± 5.63) (r =-0.456,P＜0.01;r=-0.406,P＜0.01;r=-0.542,P＜0.01).General serf-efficacy was positively correlated with cognitive work well-being and affective work well-being(r=0.382,P＜0.01;r=0.510,P＜0.01) (2) In the relationship between perceived stress and affective work well-being,general self-efficacy played a partial mediator role (P＜0.01),the mediating effect was 14.5％.In the relationship between perceived stress and cognitive work well-being,general self-efficacy played a partial mediator role (P＜0.01),and the mediating effect was 46.3％.Conclusion Perceived stress of medical stuffs influenced their work well-being through self-efficacy.It is advised to improve the medical stuffs' self-efficacy in order to raise their level of work well-being.
ABSTRACT
s: Objective To establish an HPLC method to determine the contents of ginsenoside Rg1, ginsenoside Re and ginsenoside Rb1 in Wenyang Zhenshuai Granules.Methods The separation was preformed on Agilent TC-C18 column (4.6 mm×250 mm, 5 μm). The mobile phase consisted of acetonitril-0.05% aqueous solution of phosphoric acid with gradient elution (0–35 min, 19% acetonitrile; 35–58 min, 19%–29% acetonitrile; 58–70 min, 28% acetonitrile; 70–100 min, 29%–40% acetonitrile). The flow rate was 1.0 mL/min; the wavelength of detector was 203 nm; the temperature of column was 35℃.Results The calibration curves showed good linearity in the range of 0.22–2.2 μg (ginsenoside Rg1,r=0.9998), 0.22–2.2 μg (ginsenoside Re,r=0.9998) and 0.26–2.6 μg (ginsenoside Rb1,r=0.9991), respectively. The average recoveries of ginsenoside Rg1, ginsenoside Re and ginsenoside Rb1 were 98.04%, 96.58% and 96.75%, respectively.Conclution The method is accurate, hypersensitized and reproducible, which can be applied to the quality control of Wenyang Zhenshuai Granules.
ABSTRACT
ObjectiveTo obtain stable animal models and observe Helicobacter hepaticas (Hh)colonization and pathological claracteristics,through infecting different mice strains with Hh.Methods SPF-class male BABL/c Cr,SCID/Cr and C57BL/6 Cr mice were inoculated 0.2 ml Hh standard strain ATCC51450 bacterial suspension (1 × 108CUF/ml),inoculated for 3 times with 48 hours intervals,the control group was fed with the same volume of PBS.Mice were executed at 4 weeks,8weeks and 16 weeks since last Hh inoculation,and mice esophagus,stomach,jejunum,ileum,cecum,colon,liver and pancreas tissue were taken for histopathology examination,Micro-aerobic bacteria isolation,culture and identification and Hh specific 16S rRNA gene amplification.Results The colonization rates of Hh in cecum after inoculated in BALB/c Cr mice and SCID/Cr mice at 4 weeks,8 weeks and 16 weeks were all 8/8,colonization rates in colon at 4 weeks,8 weeks and 16 weeks were 4/8,5/8,5/8 and 3/8,6/8,5/8 respectively,colonization rates in ileum and jejunum at 16 weeks were 1/8,colonization rates in liver at 8 weeks and 16 weeks were 2/8,3/8 and 2/8,2/8respectively.The colonization rates of Hh in cecum after inoculated in C57BL/6 Cr mice at 4 weeks,8weeks and 16 weeks were 1/8,2/8 and 2/8 respectively,colonization rates in colon at 8 weeks and 16weeks were 1/8,2/8 respectively.Compared with C57BL/6 Cr mice,the inflammatory changes in liver,cecum and colon were more significant in Hh infected BALB/c Cr and SCID/Cr mice (P＜0.01),and histological scores gradually increased as infection time extended (P＜0.05,P＜ 0.01 ).The histological scores were significantly higher in those with colon and liver Hh bacterial colonization than those without Hh bacterial colonization (P＜0.05).The histopathological score of cecal tissue was positively correlated with the density of Hh colonization.ConclusionDifferent mice strains are with different susceptibility to Hh,and better Hh infection model can be obtained in Hh inoculated BALB/c Cr and SCID/Cr mice.